Human Insulin is a peptide hormone produced in the islets of langerhans in the pancreas. It is central to regulating carbohydrate metabolism in mammalian cells. In synthetic medium insulin is one of the most important components in the cell culture supplement that stimulates mammalian cell growth in the absence of serum with a generation time equal to that of cells in serum-containing medium. It is recommended to be 1-20 µg/mL in media, but should be optimized for each process.
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