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Custom & Complete Media Solutions

Gemini provides complete cell culture media including sera that are customized to meet the specific needs of our customers. We can provide the same lot of serum and reagents over multiple production runs. The product can be provided with any serum concentration that our customers request. Our Customer Service Department is available to provide special quotations for the desired complete or customized media product on request.

General Information

In order to provide our customers with high quality, custom media products we need the following key information:

Media formulation or specific modification of existing formulation. Is it a proprietary formulation?

Are there any serum components included? We need to make sure that if the media requires serum, that the customer provides serum lot requirements or has the chance to test serum lots to determine which one they would like to have added.

Testing specifications: What tests are required and what are the test result specifications. Oftentimes, testing can determine the turn-around time. (pH, endotoxin, sterility, osmolality, mycoplasma, etc.)

Packaging requirements: Fill size, storage requirements.

Custom Media Orders

Gemini can tailor custom media formulations to fit all of your specific needs. Listed below are a few examples of previous custom media orders. If you have a specific custom media formulation that you need, please call 1(800)-543-6464.

DMEM: Dulbecco’s Modified Eagle’s Medium

Without Sodium Pyruvate
With HEPES buffer, High Glucose
Supports a wide variety of cell lines when using High-Glucose DMEM
Used in mammalian cell culture

RPMI 1640: Roswell Park Memorial Institute, formula 1640

Used for the culture of human normal and neoplastic leukocytes
When properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

2XBME Basal Medium

BME was originally developed for HeLa cells and mouse fibroblasts. BME has now been extended to human diploid lines such as WI-38 and MRC-5.

Penicillin:Streptomycin:Neomycin Solution

Effective against Gram positive and Gram negative bacteria
Neomycin binds to the 30S, and sometimes the 50S subunit, causing miscoding.
Neomycin inhibits initiation and protein synthesis
Calf Serum modified with Hydroxy Cholesterol
Used in mammalian transfection kits
The transfection kit increases the efficiency of transfection 100-fold or more

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